Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP206] to CD276 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CD276 antibody [SP206] - BSA and Azide free
    See all CD276 primary antibodies

  • Description

    Rabbit monoclonal [SP206] to CD276 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil, testis, stomach, stomach adenocarcinoma, lung, lung adenocarcinoma, lung SSCa, colon, colon adenocarcinoma, breast and breast ductal carcinoma tissues. Flow Cyt: HEK-293 cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab240407 is the carrier-free version of ab227670. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240407 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling CD276 with ab227670 at 1/100 dilution (2.29 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly membranous staining on the human lung carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227670 for 10 mins at room temperature. This image was generated using ab227670, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling CD276 with ab227670 at 1/100 dilution (2.29 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly membranous staining on the human breast, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227670 for 10 mins at room temperature. This image was generated using ab227670, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)

    Formalin-fixed, paraffin-embedded human stomach tissue stained for CD276 with ab227670 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227670).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)

    Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for CD276 with ab227670 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227670).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)

    Formalin-fixed, paraffin-embedded human breast tissue stained for CD276 with ab227670 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227670).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD276 with ab227670 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227670).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)

    Formalin-fixed, paraffin-embedded human colon tissue stained for CD276 with ab227670 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227670).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for CD276 with ab227670 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227670).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)

    Formalin-fixed, paraffin-embedded human lung tissue stained for CD276 with ab227670 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227670).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD276 with ab227670 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227670).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)

    Formalin-fixed, paraffin-embedded human testis tissue stained for CD276 with ab227670 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227670).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD276 with ab227670 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227670).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)

    Formalin-fixed, paraffin-embedded human lung SCCa tissue stained for CD276 with ab227670 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227670).

  • Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)
    Anti-CD276 antibody [SP206] - BSA and Azide free (ab240407)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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