Anti-Yellow Fever Virus NS1 antibody [HH7.B.D10.B8] (ab253237)
![Anti-Yellow Fever Virus NS1 antibody [HH7.B.D10.B8] (ab253237)](https://www.abcam.com/ps/products/253/ab253237/Images/ab253237-343675-AntiYellow-Fever-Virus-NS1-antibody-HH7BD10B8-elisa.jpg "Anti-Yellow Fever Virus NS1 antibody [HH7.B.D10.B8] (ab253237)")
Key features and details
- Mouse monoclonal [HH7.B.D10.B8] to Yellow Fever Virus NS1
- Suitable for: ELISA
- Isotype: IgG1
Overview
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Product name
Anti-Yellow Fever Virus NS1 antibody [HH7.B.D10.B8] -
Description
Mouse monoclonal [HH7.B.D10.B8] to Yellow Fever Virus NS1 -
Host species
Mouse -
Specificity
This antibody is specific for the NS1 protein of Yellow Fever virus, and does not cross-react with NS1 from other flaviviruses, including Dengue virus serotypes 1- 4, Zika virus, West Nile virus and Japanese Encephalitis virus. No cross-reactivity is seen with Chikungunya virus E1, E2 or C proteins.
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Tested applications
Suitable for: ELISAmore details -
Immunogen
Recombinant full length protein corresponding to Yellow fever virus Yellow Fever Virus NS1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
HH7.B.D10.B8 -
Isotype
IgG1
Images
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ELISA - Anti-Yellow Fever Virus NS1 antibody [HH7.B.D10.B8] (ab253237)
ELISA plates coated on bench overnight in 0.1M carbonate pH 9.6, 100 µl/well, washed once in wash buffer 300 µl/well (TBS + 0.1% tween 20) and blocked 2 hrs in 1% BSA in D-PBS 300 µl/well. Antibodies diluted to working strength in diluent (DPBS + 1% BSA + 0.05% Tween 20 + 0.2% Proclin 950). Added at 100 µl/well and incubated 2 hrs shaken at ambient temperature. Washed 3 x 300 µl/well. Goat anti Mouse IgG-HRP diluted 1/2500 in diluent, added at 100 µl/well and incubated with shaking 1 hr at ambient. Plate washed 6X300 µl/well. TMB added at 100 µl/well. Reaction for screening assay stopped by addition of 1M HCl 100 µl/well.
