Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [NAT105] to PD1 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IP, IHC-Fr, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PD1 antibody [NAT105] - BSA and Azide free
    See all PD1 primary antibodies

  • Description

    Mouse monoclonal [NAT105] to PD1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, WB, IP, IHC-Fr, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus corresponding to Human PD1. TY cells (human T/NK cell Leukemia).
    Database link: Q15116

  • Positive control

    • IHC-P: Human tonsil tissue.

  • General notes

    ab201811 is the carrier-free version of ab52587. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab201811 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)
    Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)

    Ab234444 staining PD1 in the MOLT-4 (human lymphoblastic leukemia T lymphoblast) treated with Ionomycin (500 ng/ml, 24 h) and PMA (10 ng/ml, 24 h) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde, permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/50). ab150117 anti-mouse IgG H&L (Alexa Fluor® 488) preadsorbed (1/100) was used as the secondary antibody. 

    ab179504 Anti-beta IV Tubulin (1/200) was used as a counter stain, detected by ab150080 AlexaFluor®594 Goat anti- Rabbit secondary (1/500)

    DAPI was used as a Nuclear counter stain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234444).

  • Flow Cytometry - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)
    Flow Cytometry - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)

    Flow cytometric analysis of MOLT-4 (human lymphoblastic leukemia cell line) cell line treated with ionomycin (500 ng/ml, 24h) and PMA (10 ng/ml, 24h) labeling PD1 with ab234444 at 1/1000 dilution (red) and an untreated control (green) compared with a Mouse monoclonal IgG1 (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234444).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PD1 with ab234444 at 1/50 dilution, followed by Rabbit Anti-Mouse IgG + Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells of human tonsil germinal center is observed. Performed on a Leica Biosystems BOND instrument. Counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234444).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)
    Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)

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