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Signal Transduction Growth Factors/Hormones Hormones

Anti-CSHL1/CSL antibody [EPR12580] (ab174295)

Price and availability

271 382 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-CSHL1/CSL antibody [EPR12580] (ab174295)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12580] to CSHL1/CSL
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-CSHL1/CSL antibody [EPR12580]
    See all CSHL1/CSL primary antibodies
  • Description

    Rabbit monoclonal [EPR12580] to CSHL1/CSL
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment within Human CSHL1/CSL. The exact sequence is proprietary.
    Database link: Q14406

  • Positive control

    • Human placenta tissue and tissue lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as CSHL1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12580
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Developmental Biology
    • Reproduction
    • Hormones

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSHL1/CSL antibody [EPR12580] (ab174295)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSHL1/CSL antibody [EPR12580] (ab174295)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CSHL1/CSL with ab174295 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-CSHL1/CSL antibody [EPR12580] (ab174295)
    Western blot - Anti-CSHL1/CSL antibody [EPR12580] (ab174295)
    Anti-CSHL1/CSL antibody [EPR12580] (ab174295) at 1/10000 dilution + Human placenta lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 25 kDa

  • Anti-CSHL1/CSL antibody [EPR12580] (ab174295)
    Anti-CSHL1/CSL antibody [EPR12580] (ab174295)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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