Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP233(2)Y] to p27 KIP 1 (phospho S10)
  • Suitable for: Dot blot, WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y]
    See all p27 KIP 1 primary antibodies

  • Description

    Rabbit monoclonal [EP233(2)Y] to p27 KIP 1 (phospho S10)

  • Host species

    Rabbit

  • Specificity

    ab62364 detects p27 KIP 1 phosphorylated at Serine 10.

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human p27 KIP 1 aa 1-100 (phospho S10) (Cysteine residue). The exact sequence is proprietary.
    Database link: P46527

  • Positive control

    • IP: MCF7 cell lysate; IHC: Human colon cancer; WB: HeLa, NIH/3T3, and C6 whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)
    Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)
    All lanes : Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
    Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa
    Observed band size: 27 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

  • Immunohistochemistry paraffin embedded sections - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)
    Immunohistochemistry paraffin embedded sections - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling p27 KIP 1 with Purified ab62364 at 1:100 dilution (4.19 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)
    Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)

    Purified ab62364 at 1/50 dilution (2µg) immunoprecipitating p27 KIP 1 in MCF7 whole cell lysate.
    Lane 1 (input): MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab62364 + MCF7 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab62364 in MCF7 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 27 kDa

  • Dot Blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)
    Dot Blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)

    Dot blot analysis of p27 KIP 1 (pS10) phospho peptide (Lane 1) and p27 KIP 1 non-phospho peptide (Lane 2) using ab62364 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.

    Blocking and Diluting buffer and concentration: 5% NFDM /TBST.

    Exposure time: 3 minutes.

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