Anti-Adipose Triglyceride Lipase (phospho S406) antibody (ab135093)
Key features and details
- Rabbit polyclonal to Adipose Triglyceride Lipase (phospho S406)
- Suitable for: ELISA, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Adipose Triglyceride Lipase (phospho S406) antibody
See all Adipose Triglyceride Lipase primary antibodies -
Description
Rabbit polyclonal to Adipose Triglyceride Lipase (phospho S406) -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Dog, Pig, Chinese hamster
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Immunogen
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Positive control
- This antibody gave a positive signal in Mouse Brown Adipose Tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab135093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ELISA Use at an assay dependent concentration. WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Target
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Function
Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. -
Tissue specificity
Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level). -
Pathway
Glycerolipid metabolism; triacylglycerol degradation. -
Involvement in disease
Note=Genetic variations in PNPLA2 may be associated with risk of diabetes mellitus type 2.
Defects in PNPLA2 are the cause of neutral lipid storage disease with myopathy (NLSDM) [MIM:610717]; also known as neutral lipid storage disease without ichthyosis. NSLDM is a neutral lipid storage disorder (NLSD) with myopathy but without ichthyosis. NLSDs are characterized by the presence of triglyceride-containing cytoplasmic droplets in leukocytes and in other tissues, including bone marrow, skin, and muscle. Individuals with NLSDM did not show obesity, in spite of a defect in triglyceride degradation in fibroblasts and in marked triglyceride storage in liver, muscles, and other visceral cells. -
Sequence similarities
Contains 1 patatin domain. -
Developmental stage
Induced during differentiation of primary preadipocytes to adipocytes. Expression increased from fetal to adult in retinal pigment epithelium. -
Cellular localization
Lipid droplet. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 66853 Mouse
- Entrez Gene: 361676 Rat
- SwissProt: Q8BJ56 Mouse
- SwissProt: P0C548 Rat
- Unigene: 29998 Mouse
- Unigene: 19196 Rat
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Alternative names
- 1110001C14Rik antibody
- Adipose triglyceride lipase antibody
- ATGL antibody
see all
Images
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Western blot - Anti-Adipose Triglyceride Lipase (phospho S406) antibody (ab135093)All lanes : Anti-Adipose Triglyceride Lipase (phospho S406) antibody (ab135093) at 1 µg/ml
Lane 1 : Mouse Brown Adipose Tissue (BAT)
Lane 2 : Mouse Brown Adipose Tissue (BAT) with Mouse Patatin-like phospholipase domain-containing protein 2 Synth (402 - 412) (Phosphorylation S 406) at 1 µg/ml
Lane 3 : Mouse Brown Adipose Tissue (BAT) with Mouse Patatin-like phospholipase domain-containing protein 2 Synth (402 - 413) (No Modifications) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 35 kDa (possible non-specific binding), 85 kDa (possible non-specific binding)
Exposure time: 20 minutes -
ELISA - Anti-Adipose Triglyceride Lipase (phospho S406) antibody (ab135093)ab135093 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.
References (11)
ab135093 has been referenced in 11 publications.
- Tarabra E et al. The omentum of obese girls harbors small adipocytes and browning transcripts. JCI Insight 5:N/A (2020). PubMed: 32125283
- Yin C et al. PID1 alters the antilipolytic action of insulin and increases lipolysis via inhibition of AKT/PKA pathway activation. PLoS One 14:e0214606 (2019). PubMed: 30990811
- Zhou J et al. A Liver-Specific Thyromimetic, VK2809, Decreases Hepatosteatosis in Glycogen Storage Disease Type Ia. Thyroid 29:1158-1167 (2019). PubMed: 31337282
- Cao Q et al. Carotid baroreceptor stimulation in obese rats affects white and brown adipose tissues differently in metabolic protection. J Lipid Res 60:1212-1224 (2019). PubMed: 31126973
- Zhou J et al. Thyroid Hormone Status Regulates Skeletal Muscle Response to Chronic Motor Nerve Stimulation. Front Physiol 10:1363 (2019). PubMed: 31736784
Images
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Western blot - Anti-Adipose Triglyceride Lipase (phospho S406) antibody (ab135093)All lanes : Anti-Adipose Triglyceride Lipase (phospho S406) antibody (ab135093) at 1 µg/ml
Lane 1 : Mouse Brown Adipose Tissue (BAT)
Lane 2 : Mouse Brown Adipose Tissue (BAT) with Mouse Patatin-like phospholipase domain-containing protein 2 Synth (402 - 412) (Phosphorylation S 406) at 1 µg/ml
Lane 3 : Mouse Brown Adipose Tissue (BAT) with Mouse Patatin-like phospholipase domain-containing protein 2 Synth (402 - 413) (No Modifications) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 35 kDa (possible non-specific binding), 85 kDa (possible non-specific binding)
Exposure time: 20 minutes
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ELISA - Anti-Adipose Triglyceride Lipase (phospho S406) antibody (ab135093)
ab135093 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.
