Anti-GKN2 antibody [EPR15376(B)] - BSA and Azide free (ab250722)
![Anti-GKN2 antibody [EPR15376(B)] - BSA and Azide free (ab250722)](https://www.abcam.com/ps/products/250/ab250722/Images/ab250722-406075-anti-gkn2-antibody-epr15376-b-bsa-and-azide-free-western-blot.jpg "Anti-GKN2 antibody [EPR15376(B)] - BSA and Azide free (ab250722)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15376(B)] to GKN2 - BSA and Azide free
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
Overview
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Product name
Anti-GKN2 antibody [EPR15376(B)] - BSA and Azide free
See all GKN2 primary antibodies -
Description
Rabbit monoclonal [EPR15376(B)] to GKN2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250722 is the carrier-free version of ab184163 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250722 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Gastrokine 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15376(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GKN2 antibody [EPR15376(B)] - BSA and Azide free (ab250722)Anti-GKN2 antibody [EPR15376(B)] (ab184163) at 1/50000 dilution + Human stomach lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDaThis data was developed using ab184163, the same antibody clone in a different buffer formulation.
The observed MW is lower than the predicted 20kDa, which may be due to the removing of a 20 amino acid signal peptide.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15376(B)] - BSA and Azide free (ab250722)](https://www.abcam.com/ps/products/250/ab250722/Images/ab250722-2-anti-gkn2-antibody-epr15376-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15376(B)] - BSA and Azide free (ab250722)This data was developed using ab184163, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling GKN2 with ab184163 at 1/500 dilution. The slide is counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Immunoprecipitation - Anti-GKN2 antibody [EPR15376(B)] - BSA and Azide free (ab250722)](https://www.abcam.com/ps/products/250/ab250722/Images/ab250722-1-anti-gkn2-antibody-epr15376-b-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GKN2 antibody [EPR15376(B)] - BSA and Azide free (ab250722)This data was developed using ab184163, the same antibody clone in a different buffer formulation.Immunoprecipitation of Human stomach lysates (lane 1) or PBS (lane 2) using ab184163. Detection of GKN2 utilised ab184163 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution. -
![Anti-GKN2 antibody [EPR15376(B)] - BSA and Azide free (ab250722)](https://www.abcam.com/ps/products/250/ab250722/Images/ab250722-4-benefits-of-recombinant-antibodies.png)
Anti-GKN2 antibody [EPR15376(B)] - BSA and Azide free (ab250722)