Porcine IFN beta ELISA Kit (ab273222)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 1.64 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Overview
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Product name
Porcine IFN beta ELISA Kit
See all Interferon beta kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2 pg/ml -
Range
1.64 pg/ml - 400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 90.69 88% - 95% Serum 86.51 82% - 89% Plasma 71.62 68% - 75% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Pig -
Product overview
Porcine IFN beta ELISA Kit (ab273222) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine IFN beta in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Porcine IFN-beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN-beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Porcine IFN-beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN-beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Porcine IFN beta coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine IFN beta Antibody 2 vials Porcine IFN beta Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Has antiviral, antibacterial and anticancer activities. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- beta-interferon
- Fibroblast interferon
- IFB
see all
Images
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Porcine IFN beta Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Porcine IFN beta ELISA kit (ab273222) Standard curve.