Anti-TIMP1 antibody [EPR16616] - BSA and Azide free (ab232937)
![Anti-TIMP1 antibody [EPR16616] - BSA and Azide free (ab232937)](https://www.abcam.com/ps/products/232/ab232937/Images/ab232937-304305-anti-timp1-antibody-epr16616-western-blot.jpg "Anti-TIMP1 antibody [EPR16616] - BSA and Azide free (ab232937)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16616] to TIMP1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Overview
-
Product name
Anti-TIMP1 antibody [EPR16616] - BSA and Azide free
See all TIMP1 primary antibodies -
Description
Rabbit monoclonal [EPR16616] to TIMP1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Mouse lung culture supernatant (7 days) and leg bone lysate.
-
General notes
Ab232937 is the carrier-free version of ab179580. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab232937 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16616 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-TIMP1 antibody [EPR16616] - BSA and Azide free (ab232937)All lanes : Anti-TIMP1 antibody [EPR16616] - BSA and Azide free (ab232937) at 1/1000 dilution
Lane 1 : Mouse lung culture supernatant (7 days) at 20 µl
Lane 2 : Mouse leg bone lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Blocking / Dilution: 5% NFDM/TBST
Exposure time: Lane 1: 32 seconds; Lane 2: 3 minutes
The molecular mass observed is consistent with the literature (PMID: 17202148).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179580).
-
![Anti-TIMP1 antibody [EPR16616] - BSA and Azide free (ab232937)](https://www.abcam.com/ps/products/232/ab232937/Images/ab232937-2-benefits-of-recombinant-antibodies.png)
Anti-TIMP1 antibody [EPR16616] - BSA and Azide free (ab232937)
