Anti-TWEAK antibody (ab37170)
Key features and details
- Rabbit polyclonal to TWEAK
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-TWEAK antibody
See all TWEAK primary antibodies -
Description
Rabbit polyclonal to TWEAK -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human TWEAK.
Database link: O43508
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAK antibody (ab37170)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse brain tissue staining TWEAK with ab37170 at 20µg/ml
-
Western blot - Anti-TWEAK antibody (ab37170)Western Blot of HeLa cell lysate labeling TWEAK with Anti-TWEAK antibody (ab37170) at (1) 1 and (2) 2µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAK antibody (ab37170)Immunohistochemistry analysis Formalin/PFA-fixed paraffin-embedded human brain tissue sections labeling TWEAK with ab37170 at 10 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAK antibody (ab37170)Immunohistochemistry analysis Formalin/PFA-fixed paraffin-embedded mouse brain tissue sections labeling TWEAK with ab37170 at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. -
Western blot - Anti-TWEAK antibody (ab37170)Lane 1 : Anti-TWEAK antibody (ab37170) at 1 µg/ml
Lane 2 : Anti-TWEAK antibody (ab37170) at 2 µg/ml
All lanes : Mouse pancreas tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 27,36 kDaPrimary incubation: 1h incubation at RT in 5% NFDM/TBST
-
Western blot - Anti-TWEAK antibody (ab37170)Anti-TWEAK antibody (ab37170) at 2 µg/ml + Mouse Liver Tissue Lysates at 15 µg
Secondary
Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 27,36 kDaPrimary incubation time: 1h incubation at RT in 5% NFDM/TBST.
-
Western blot - Anti-TWEAK antibody (ab37170)All lanes : Anti-TWEAK antibody (ab37170) at 1 µg/ml
Lane 1 : Recombinant human TWEAK at 0.005 µg
Lane 2 : Recombinant human TWEAK at 0.025 µg
Lane 3 : Recombinant human TWEAK at 0.05 µg
Predicted band size: 27,36 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
TWEAK migrates at aberrant positions in SDS-PAGE; hence the observed band is different from the predicted molecular weight of 27 kDa.
