Anti-Histone H4 (formyl K91) antibody [EPR18083] - BSA and Azide free (ab250005)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18083] to Histone H4 (formyl K91) - BSA and Azide free
- Suitable for: PepArr, WB
- Reacts with: Mouse
Overview
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Product name
Anti-Histone H4 (formyl K91) antibody [EPR18083] - BSA and Azide free
See all Histone H4 primary antibodies -
Description
Rabbit monoclonal [EPR18083] to Histone H4 (formyl K91) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species PepArr HumanWB Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250005 is the carrier-free version of ab177862. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250005 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18083 -
Isotype
IgG
Images
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Anti-Histone H4 (formyl K91) antibody [EPR18083] (ab177862) at 1/500 dilution + NIH/3T3 (Mouse embyro fibroblast cells) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 5 secondsThis data was developed using ab177862, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% BSA/TBST.
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This data was developed using ab177862, the same antibody clone in a different buffer formulation.ab177862 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity. The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here. -