Anti-Cytokeratin 9 antibody [EPR10932] - BSA and Azide free (ab249416)
![Anti-Cytokeratin 9 antibody [EPR10932] - BSA and Azide free (ab249416)](https://www.abcam.com/ps/products/249/ab249416/Images/ab249416-403375-anti-cytokeratin-9-antibody-epr10932-bsa-and-azide-free-western-blot-human.jpg "Anti-Cytokeratin 9 antibody [EPR10932] - BSA and Azide free (ab249416)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10932] to Cytokeratin 9 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Cytokeratin 9 antibody [EPR10932] - BSA and Azide free
See all Cytokeratin 9 primary antibodies -
Description
Rabbit monoclonal [EPR10932] to Cytokeratin 9 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment within Human Cytokeratin 9. The exact sequence is proprietary.
Database link: P35527 -
General notes
Ab249416 is the carrier-free version of ab167398. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249416 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR10932 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cytokeratin 9 antibody [EPR10932] - BSA and Azide free (ab249416)All lanes : Anti-Cytokeratin 9 antibody [EPR10932] (ab167398) at 1/10000 dilution
Lane 1 : NCI-H460 (Human Large Cell Lung Cancer epithelial cell) whole cell lysates
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDaThis data was developed using ab167398, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-3: 30 seconds; Lane 4: 180 seconds.
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![Anti-Cytokeratin 9 antibody [EPR10932] - BSA and Azide free (ab249416)](https://www.abcam.com/ps/products/249/ab249416/Images/ab249416-2-benefits-of-recombinant-antibodies.png)
Anti-Cytokeratin 9 antibody [EPR10932] - BSA and Azide free (ab249416)
