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Anti-C14orf169 / NO66 antibody [EPR18656] - BSA and Azide free (ab251154)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18656] to C14orf169 / NO66 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-C14orf169 / NO66 antibody [EPR18656] - BSA and Azide free
    See all C14orf169 / NO66 primary antibodies

  • Description

    Rabbit monoclonal [EPR18656] to C14orf169 / NO66 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251154 is the carrier-free version of ab192861. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251154 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] - BSA and Azide free (ab251154)
    Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] - BSA and Azide free (ab251154)
    All lanes : Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
    Lane 4 : NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 5 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
    Lane 6 : LLC (Mouse lung carcinoma cell line) whole cell lysate
    Lane 7 : mESC (Mouse embryonic stem cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 71 kDa
    Observed band size: 71 kDa


    Exposure time: 3 minutes


    This data was developed using ab192861, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The predicted MW of human's, mouse's and rat's NO66 are 71kDa, 68kDa and 67kDa, respectively.

  • Immunoprecipitation - Anti-C14orf169 / NO66 antibody [EPR18656] - BSA and Azide free (ab251154)
    Immunoprecipitation - Anti-C14orf169 / NO66 antibody [EPR18656] - BSA and Azide free (ab251154)
    This data was developed using ab192861, the same antibody clone in a different buffer formulation.C14orf169 / NO66 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate with ab192861 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab192861 at 1/1000 dilution. VeriBlot for IP Detection Reagent(HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HEK-293 whole cell lysate, 10µg (Input). Lane 2: ab192861 IP in HEK-293 whole cell lysate. Lane 3: Rabbit IgG,monoclonal [EPR18656]-Isotype Control (ab172730) instead of ab192861 in HEK-293 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes.
  • Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] - BSA and Azide free (ab251154)
    Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] - BSA and Azide free (ab251154)
    All lanes : Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 71 kDa
    Observed band size: 71 kDa


    Exposure time: 3 minutes


    This data was developed using ab192861, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] - BSA and Azide free (ab251154)
    Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] - BSA and Azide free (ab251154)
    All lanes : Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861) at 1/200 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Mouse lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 71 kDa
    Observed band size: 71 kDa


    Exposure time: 3 minutes


    This data was developed using ab192861, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-C14orf169 / NO66 antibody [EPR18656] - BSA and Azide free (ab251154)
    Anti-C14orf169 / NO66 antibody [EPR18656] - BSA and Azide free (ab251154)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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