Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NG2 antibody [EPR22410-145] - BSA and Azide free (ab256351)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22410-145] to NG2 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-NG2 antibody [EPR22410-145] - BSA and Azide free
    See all NG2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22410-145] to NG2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A375 and SK-MEL-28 whole cell lysate. IHC-P: Human melanoma tissue. ICC/IF: A375 cells. Flow Cyt: A375 cells. IP: A375 whole cell lysate.

  • General notes

    Ab256351 is the carrier-free version of ab255811. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256351 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR22410-145] - BSA and Azide free (ab256351)
    Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR22410-145] - BSA and Azide free (ab256351)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A375 (human malignant melanoma epithelial cell) cells labeling NG2 with ab255811 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in A375 cells. Negative control: SK-BR-3 (PMID: 20852124). The nuclear counterstain is DAPI (blue). Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255811).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR22410-145] - BSA and Azide free (ab256351)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR22410-145] - BSA and Azide free (ab256351)

    Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling NG2 with ab255811 at 1/100 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human melanoma (PMID: 25197555). The section was incubated with ab255811 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255811).
  • Flow Cytometry - Anti-NG2 antibody [EPR22410-145] - BSA and Azide free (ab256351)
    Flow Cytometry - Anti-NG2 antibody [EPR22410-145] - BSA and Azide free (ab256351)

    Flow cytometric analysis of SK-BR-3 (Human breast adenocarcinoma epithelial cell, Left panel) / A375 (Human malignant melanoma epithelial cell, Right panel) labeling NG2 with ab255811 at 1/600 (red) compared with a Rabbit monoclonal IgG (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Negative control: SK-BR-3 (PMID: 20852124). Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255811).
  • Immunoprecipitation - Anti-NG2 antibody [EPR22410-145] - BSA and Azide free (ab256351)
    Immunoprecipitation - Anti-NG2 antibody [EPR22410-145] - BSA and Azide free (ab256351)

    NG2 was immunoprecipitated from 0.35 mg A375 (Human malignant melanoma epithelial cell) whole cell lysate with ab255811 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab255811 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.

    Lane 1: A375 whole cell lysate 10 µg (Input). 
    Lane 2: ab255811 IP in A375 whole cell lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255811 in A375 whole cell lysate.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 9477982, 8790396). A truncated form is also detected (190kDa, PMID: 25387269).

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255811).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR22410-145] - BSA and Azide free (ab256351)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR22410-145] - BSA and Azide free (ab256351)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling NG2 with ab255811 at 1/100 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in human colon. (PMID: 25197555). The section was incubated with ab255811 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255811).
  • Anti-NG2 antibody [EPR22410-145] - BSA and Azide free (ab256351)
    Anti-NG2 antibody [EPR22410-145] - BSA and Azide free (ab256351)

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