Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPP1R1A antibody [EP902Y] - BSA and Azide free (ab247299)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP902Y] to PPP1R1A - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PPP1R1A antibody [EP902Y] - BSA and Azide free
    See all PPP1R1A primary antibodies

  • Description

    Rabbit monoclonal [EP902Y] to PPP1R1A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247299 is the carrier-free version of ab40877. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247299 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PPP1R1A antibody [EP902Y] - BSA and Azide free (ab247299)
    Western blot - Anti-PPP1R1A antibody [EP902Y] - BSA and Azide free (ab247299)
    Anti-PPP1R1A antibody [EP902Y] (ab40877) at 1/40000 dilution + Rat brain tissue lysate at 10 µg

    Predicted band size: 19 kDa
    Observed band size: 27 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab40877, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1R1A antibody [EP902Y] - BSA and Azide free (ab247299)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1R1A antibody [EP902Y] - BSA and Azide free (ab247299)
    This data was developed using ab40877, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human normal brain tissue using ab40877 diluted 1:100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-PPP1R1A antibody [EP902Y] - BSA and Azide free (ab247299)
    Immunocytochemistry - Anti-PPP1R1A antibody [EP902Y] - BSA and Azide free (ab247299)

    This data was developed using ab40877, the same antibody clone in a different buffer formulation.ICC/IF image of ab40877 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40877, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry - Anti-PPP1R1A antibody [EP902Y] - BSA and Azide free (ab247299)
    Flow Cytometry - Anti-PPP1R1A antibody [EP902Y] - BSA and Azide free (ab247299)
    This data was developed using ab40877, the same antibody clone in a different buffer formulation.Overlay histogram showing SH-SY5Y cells stained with ab40877 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40877, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Anti-PPP1R1A antibody [EP902Y] - BSA and Azide free (ab247299)
    Anti-PPP1R1A antibody [EP902Y] - BSA and Azide free (ab247299)

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