Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998)
![Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998)](https://www.abcam.com/ps/products/105/ab105998/Images/ab105998-118372-anti-pdcd4-phospho-s457-antibody-9g6-western-blot.jpg "Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998)")
Key features and details
- Mouse monoclonal [9G6] to PDCD4 (phospho S457)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-PDCD4 (phospho S457) antibody [9G6]
See all PDCD4 primary antibodies -
Description
Mouse monoclonal [9G6] to PDCD4 (phospho S457) -
Host species
Mouse -
Specificity
ab105998 is specific for Human PDCD4 protein phosphorylated at Ser457. Cross-reactivity with PDCD4 from other sources has not been determined. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis
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Immunogen
Synthetic peptide corresponding to residues surrounding Ser457 of Human PDCD4 (NP_055271).
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Positive control
- NIH-3T3 cells stimulated with PDGF, Recombinant PDCD4 protein, Human small intestine tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from concentrated tissue culture supernate by Protein A chromatography. -
Clonality
Monoclonal -
Clone number
9G6 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998)All lanes : Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998) at 1/2000 dilution
Lane 1 : NIH-3T3 cells stimulated with 30 ng/ml PDGF (5 min treatment)
Lane 2 : Unstimulated (non-phosphorylated) NIH 3T3 cells
Secondary
All lanes : HRP conjugated Rabbit anti-Mouse IgG at 1/2000 dilution
Predicted band size: 62 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998)](https://www.abcam.com/ps/products/105/ab105998/Images/ab105998-118371-anti-pdcd4-phospho-s457-antibody-9g6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998)ab105998 at 1/1000 dilution staining PDCD4 in Human small intestine by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
The image shows the localization of ab105998 as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
