Human PIK3R2 (PI 3 Kinase p85 beta) knockout HEK-293T cell lysate (ab257586)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260295 - Human PIK3R2 knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human PIK3R2 (PI 3 Kinase p85 beta) knockout HEK293T cell lysate (ab257586)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: PIK3R2 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab28356 observed at 85 kDa. Red - loading control ab181602 observed at 37 kDa.
ab28356 Anti-PI 3 Kinase p85 beta antibody [T15] was shown to specifically react with PI 3 Kinase p85 beta in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266799 (knockout cell lysate ab257586) was used. Wild-type and PI 3 Kinase p85 beta knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab28356 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human PIK3R2 (PI 3 Kinase p85 beta) knockout HEK293T cell lysate (ab257586)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: PIK3R2 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab180967 observed at 85 kDa. Red - loading control ab8245 observed at 37 kDa.
ab180967 Anti-PI 3 Kinase p85 beta antibody [EPR18416] was shown to specifically react with PI 3 Kinase p85 beta in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266799 (knockout cell lysate ab257586) was used. Wild-type and PI 3 Kinase p85 beta knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180967 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human PIK3R2 knockout HEK293T cell lysate (ab257586)Homozygous: 1 bp insertion in exon 2