Anti-ERp57 antibody (ab264383)
Key features and details
- Rabbit polyclonal to ERp57
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ERp57 antibody
See all ERp57 primary antibodies -
Description
Rabbit polyclonal to ERp57 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human ERp57 aa 25-75. The exact sequence is proprietary.
Database link: P30101 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK and NIH/3T3 whole cell lysate. IHC-P: Human colon carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ERp57 antibody (ab264383)All lanes : Anti-ERp57 antibody (ab264383) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 3 secondsLysates prepared using NETN lysis buffer.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody (ab264383)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling ERp57 with ab264383 at 1/5000 dilution. Detection: DAB.
Epitope retrieval with citrate buffer pH 6.0.
