Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10679(B)] to ERp57 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free
    See all ERp57 primary antibodies

  • Description

    Rabbit monoclonal [EPR10679(B)] to ERp57 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249093 is the carrier-free version of ab154197. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249093 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR10679(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)
    Western blot - Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)

    This data was developed using ab154197, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: ERp57 knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HepG2 whole cell lysate (20 µg)

    Lane 4: Hek293 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab154197 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab154197 was shown to specifically react with ERp57 in wild-type HAP1 cells as signal was lost in ERp57 knockout cells. Wild-type and ERp57 knockout samples were subjected to SDS-PAGE. ab154197 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)

    This data was developed using ab154197, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling ERp57 with ab154197 at 1/50 dilution Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)
    Western blot - Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)
    All lanes : Anti-ERp57 antibody [EPR10679(B)] (ab154197) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 57 kDa



    This data was developed using ab154197, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)
    This data was developed using ab154197, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling ERp57 with ab154197 at 1/50 dilution Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)
    This data was developed using ab154197, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154197 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)
    This data was developed using ab154197, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154197 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)
    This data was developed using ab154197, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154197 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)
    Anti-ERp57 antibody [EPR10679(B)] - BSA and Azide free (ab249093)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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