Anti-eIF4B antibody - N-terminal (ab228800)
Key features and details
- Rabbit polyclonal to eIF4B - N-terminal
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4B antibody - N-terminal
See all eIF4B primary antibodies -
Description
Rabbit polyclonal to eIF4B - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Rhesus monkey -
Immunogen
Recombinant fragment within Human eIF4B (N terminal). The exact sequence is proprietary.
Database link: P23588 -
Positive control
- WB: NTERA-2 cl.D1 [NT2/D1], IMR32, U-87 MG and MCF7 whole cell lysats; HEK-293T, A431, HeLa, HepG2, SK-N-SH, IMR32 and SK-N-AS whole cell extracts; eIF4B-transfected HEK-293T whole cell extract. IHC-P: Mouse kidney tissue. ICC/IF: MCF7 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-eIF4B antibody - N-terminal (ab228800) at 1/1000 dilution
Lane 1 : NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 2 : IMR32 whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 69 kDa7.5% SDS-PAGE gel.
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Paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for eIF4B (green) using ab228800 at 1/500 dilution in ICC/IF.
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Paraffin-embedded mouse kidney stained for eIF4B using ab228800 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-eIF4B antibody - N-terminal (ab228800) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 69 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-eIF4B antibody - N-terminal (ab228800) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 69 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-eIF4B antibody - N-terminal (ab228800) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : eIF4B-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 69 kDa7.5% SDS-PAGE gel.