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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-eIF4B antibody - N-terminal (ab228800)

Anti-eIF4B antibody - N-terminal (ab228800)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to eIF4B - N-terminal
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-eIF4B antibody - N-terminal
    See all eIF4B primary antibodies
  • Description

    Rabbit polyclonal to eIF4B - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human eIF4B (N terminal). The exact sequence is proprietary.
    Database link: P23588

  • Positive control

    • WB: NTERA-2 cl.D1 [NT2/D1], IMR32, U-87 MG and MCF7 whole cell lysats; HEK-293T, A431, HeLa, HepG2, SK-N-SH, IMR32 and SK-N-AS whole cell extracts; eIF4B-transfected HEK-293T whole cell extract. IHC-P: Mouse kidney tissue. ICC/IF: MCF7 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation

Images

  • Western blot - Anti-eIF4B antibody - N-terminal (ab228800)
    Western blot - Anti-eIF4B antibody - N-terminal (ab228800)
    All lanes : Anti-eIF4B antibody - N-terminal (ab228800) at 1/1000 dilution

    Lane 1 : NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 2 : IMR32 whole cell lysate
    Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 69 kDa



    7.5% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4B antibody - N-terminal (ab228800)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF4B antibody - N-terminal (ab228800)

    Paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for eIF4B (green) using ab228800 at 1/500 dilution in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B antibody - N-terminal (ab228800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B antibody - N-terminal (ab228800)

    Paraffin-embedded mouse kidney stained for eIF4B using ab228800 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-eIF4B antibody - N-terminal (ab228800)
    Western blot - Anti-eIF4B antibody - N-terminal (ab228800)
    All lanes : Anti-eIF4B antibody - N-terminal (ab228800) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 69 kDa



    7.5% SDS-PAGE gel.

  • Western blot - Anti-eIF4B antibody - N-terminal (ab228800)
    Western blot - Anti-eIF4B antibody - N-terminal (ab228800)
    All lanes : Anti-eIF4B antibody - N-terminal (ab228800) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
    Lane 3 : IMR32 whole cell extract
    Lane 4 : SK-N-AS whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 69 kDa



    7.5% SDS-PAGE gel.

  • Western blot - Anti-eIF4B antibody - N-terminal (ab228800)
    Western blot - Anti-eIF4B antibody - N-terminal (ab228800)
    All lanes : Anti-eIF4B antibody - N-terminal (ab228800) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : eIF4B-transfected HEK-293T whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 69 kDa



    7.5% SDS-PAGE gel.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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