Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

200 µl 274 732 ₸

Order now and get it on Thursday February 25, 2021

Key features and details

Product name

Anti-eIF4B antibody
See all eIF4B primary antibodies
Description

Rabbit polyclonal to eIF4B
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human eIF4B aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as ab50102)
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK293 (Human embryonic kidney cell line) MCF7 (Human breast adenocarcinoma cell line)

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

Batches of this product that have a concentration
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Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab50103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
WB	Human
All applications	Mouse Rat Cow

Application	Abreviews	Notes
WB		1/250. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).

Notes
WB 1/250. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).

Function

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Sequence similarities

Contains 1 RRM (RNA recognition motif) domain.
Post-translational
modifications

Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
Target information above from: UniProt accession P23588 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 505850 Cow
- Entrez Gene: 1975 Human
- Entrez Gene: 75705 Mouse
- Entrez Gene: 300253 Rat
- Omim: 603928 Human
- SwissProt: P23588 Human
- SwissProt: Q8BGD9 Mouse
- Unigene: 648394 Human
- Unigene: 702041 Human
- Unigene: 290022 Mouse
- Unigene: 391795 Mouse
- Unigene: 95954 Rat
see all
Alternative names
- 2310046H11Rik antibody
- AL024095 antibody
- C85189 antibody
- EIF 4B antibody
- eIF-4B antibody
- EIF4B antibody
- Eukaryotic initiation factor 4B antibody
- Eukaryotic translation initiation factor 4B antibody
- IF4B_HUMAN antibody
- PRO1843 antibody
see all

Publishing research using ab50103? Please let us know so that we can cite the reference in this datasheet.

ab50103 has been referenced in 1 publication.

Domingo-Gil E et al. Identification of cellular genes induced in human cells after activation of the OAS/RNaseL pathway by vaccinia virus recombinants expressing these antiviral enzymes. J Interferon Cytokine Res 30:171-88 (2010). PubMed: 20038200