Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [1F5] to eIF4B
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Mouse, Human, Recombinant fragment
Isotype: IgG2b

Product name

Anti-eIF4B antibody [1F5]
See all eIF4B primary antibodies
Description

Mouse monoclonal [1F5] to eIF4B
Host species

Mouse
Tested applications

Suitable for: WB, Flow Cyt, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Human, Recombinant fragment
Immunogen

Purified recombinant fragment of Human eIF4B expressed in E. Coli.
Positive control
- A549, A431, HepG2, HEK293, Jurkat, K562 and MCF-7 cell lysates. HeLa and NIH3T3 cells.
General notes

This product was changed from ascites to supernatant. Lot no’s high than GR185156-5 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

1F5
Isotype

IgG2b
Research areas

Western blot - Anti-eIF4B antibody [1F5] (ab129369)

All lanes : Anti-eIF4B antibody [1F5] (ab129369) at 1/500 dilution

Lane 1 : A549 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HEK293 cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : K562 cell lysate
Lane 8 : NIH3T3 cell lysate
Lane 9 : MCF-7 cell lysate

Predicted band size: 69 kDa
Flow Cytometry - Anti-eIF4B antibody [1F5] (ab129369)

ab129369 at 1/200 dilution (green) or negative control (red) staining eIF4B in HeLa cells by flow cytometry.
Immunocytochemistry/ Immunofluorescence - Anti-eIF4B antibody [1F5] (ab129369)

ab129369 at 1/200 dilution staining eIF4B in NIH3T3 cells by immunofluorescence. DNA was stained with DRAQ5 dye (blue) and actin with Alexa 555 phalloidin (red).
Western blot - Anti-eIF4B antibody [1F5] (ab129369)

Anti-eIF4B antibody [1F5] (ab129369) at 1/500 dilution + eIF4B recombinant fragment (aa 381-585)

Predicted band size: 69 kDa

The expected molecular weight of the recombinant fragment is 48 kDa.

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