Anti-PGK1 antibody (ab154613)
Key features and details
- Rabbit polyclonal to PGK1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-PGK1 antibody
See all PGK1 primary antibodies -
Description
Rabbit polyclonal to PGK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae -
Immunogen
A recombinant fragment corresponding to a region within amino acids 1-217 of Human PGK1 (Uniprot ID P00558).
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Positive control
- A549, HeLa, mouse brain and rat liver whole cell lysates. Yeast wild type 303. HeLa cells. Human hepatoma tissue.
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General notes
Store as a concentrated solution.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PGK1 antibody (ab154613) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE -
Anti-PGK1 antibody (ab154613) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 45 kDa
10% SDS PAGE -
All lanes : Anti-PGK1 antibody (ab154613) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate (untreated)
Lane 2 : HeLa whole cell lysate (1% O2 treatment for 24hr)
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE -
Anti-PGK1 antibody (ab154613) at 1/1000 dilution + Rat liver whole cell lysate at 50 µg
Predicted band size: 45 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGK1 antibody (ab154613)
Immunohistochemical analysis of paraffin-embedded human Hepatoma, labeling PGK1 using ab154613 at 1/500 dilution.
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Immunofluorescence analysis of methanol-fixed HeLa cells, labeling PGK1 using ab154613 at 1/50 dilution. Right panel costained with Hoechst 33342.