Anti-XAF1 antibody (ab81353)
Key features and details
- Rabbit polyclonal to XAF1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-XAF1 antibody
See all XAF1 primary antibodies -
Description
Rabbit polyclonal to XAF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminal of human XAF1, identical to the related rat and mouse sequence.
-
Positive control
- WB: HepG2 cell lysate IHC-P: Human Endometrial Carcinoma Tissue ICC: MCF7 cells
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XAF1 antibody (ab81353)
ab81353 at 1µg/ml staining XAF1 in Human endometrium cancer tissue sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded sections). The tissue sections underwent antigen retrieval by heat. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.
-
Western blot - Anti-XAF1 antibody (ab81353)All lanes :
All lanes : HEPG2 whole cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 35 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-XAF1 antibody (ab81353)ICC/IF image of ab81353 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81353, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
