Anti-APP-1 antibody (ab241616)
Key features and details
- Rabbit polyclonal to APP-1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-APP-1 antibody
See all APP-1 primary antibodies -
Description
Rabbit polyclonal to APP-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human APP-1 aa 525-575. The exact sequence is proprietary. (NP_003810.1).
Database link: Q13310 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 cell lysate. IP: HeLa cell lysate. IHC-P: Human prostate carcinoma and colon carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to APP-1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-APP-1 antibody (ab241616)All lanes : Anti-APP-1 antibody (ab241616) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 71 kDa
Exposure time: 10 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APP-1 antibody (ab241616)Formalin-fixed, paraffin-embedded human prostate carcinoma (left) and colon carcinoma (right) tissue stained for APP-1 using ab241616 at 1/200 dilution in immunohistochemical analysis. DAB staining.
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Immunoprecipitation - Anti-APP-1 antibody (ab241616)APP-1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241616 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241616 at 1 µg/ml.
Lane 1: ab241616 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 3 seconds.
