Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD1c antibody [EPR23189-305] - BSA and Azide free (ab273060)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23189-305] to CD1c - BSA and Azide free
  • Suitable for: Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CD1c antibody [EPR23189-305] - BSA and Azide free
    See all CD1c primary antibodies

  • Description

    Rabbit monoclonal [EPR23189-305] to CD1c - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Jurkat cells; Human PBMCs.

  • General notes

    Note: Clone EPR23189-305 (this product) is a different clone to that of ab246520.

    ab273060 is the carrier-free version of ab273049. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23189-305

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-CD1c antibody [EPR23189-305] - BSA and Azide free (ab273060)
    Flow Cytometry - Anti-CD1c antibody [EPR23189-305] - BSA and Azide free (ab273060)

    Flow cytometry overlay histogram histogram showing wild-type (green line) and CD1C knockout Jurkat cells stained with ab273049 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab273049) (1x106 in 100 µl at 0.2 µg/ml) for 30 min at 4°C.

    The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150081) was used at 1/2000 for 30 min at 4°C.

    Isotype control antibody was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody (wild-type Jurkat - black line; CD1C knockout Jurkat - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  • Flow Cytometry - Anti-CD1c antibody [EPR23189-305] - BSA and Azide free (ab273060)
    Flow Cytometry - Anti-CD1c antibody [EPR23189-305] - BSA and Azide free (ab273060)

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD1c with ab273049 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Cells were stained with rabbit IgG (Left) or ab273049 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273049).

  • Flow Cytometry - Anti-CD1c antibody [EPR23189-305] - BSA and Azide free (ab273060)
    Flow Cytometry - Anti-CD1c antibody [EPR23189-305] - BSA and Azide free (ab273060)

    Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte)(Left) / Jurkat (Human T cell leukemia T lymphocyte)(Right) cells labelling CD1c with ab273049 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: THP-1 (PMID: 24935257).

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273049).

  • Anti-CD1c antibody [EPR23189-305] - BSA and Azide free (ab273060)
    Anti-CD1c antibody [EPR23189-305] - BSA and Azide free (ab273060)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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