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Anti-Lck (phospho Y505) antibody [LckY505-A3] (ab278722)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [LckY505-A3] to Lck (phospho Y505)
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Lck (phospho Y505) antibody [LckY505-A3]
    See all Lck primary antibodies

  • Description

    Rabbit monoclonal [LckY505-A3] to Lck (phospho Y505)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Lck (phospho Y505). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P06239

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Jurkat (treated with Calyculin A) cell extract, Flow cyt: Daudi cells (treated with IFNa + IL-4 + pervanadate ).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Lck (phospho Y505) antibody [LckY505-A3] (ab278722)
    Western blot - Anti-Lck (phospho Y505) antibody [LckY505-A3] (ab278722)
    All lanes : Anti-Lck (phospho Y505) antibody [LckY505-A3] (ab278722) at 0.1 µg/ml

    Lane 1 : Jurkat cell extract treated with 200nM calyculin A
    Lane 2 : Jurkat cell extract untreated with 200nM calyculin A

    Predicted band size: 58 kDa

  • Flow Cytometry - Anti-Lck (phospho Y505) antibody [LckY505-A3] (ab278722)
    Flow Cytometry - Anti-Lck (phospho Y505) antibody [LckY505-A3] (ab278722)
    Flow cytometric analysis of Daudi cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) using ab278722 at 1 µg/mL.
  • Flow Cytometry - Anti-Lck (phospho Y505) antibody [LckY505-A3] (ab278722)
    Flow Cytometry - Anti-Lck (phospho Y505) antibody [LckY505-A3] (ab278722)
    Peptide blocking flow cytometric analysis of Daudi cells secondary antibody only negative control (light blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using ab278722 at 1 µg/mL.
  • Western blot - Anti-Lck (phospho Y505) antibody [LckY505-A3] (ab278722)
    Western blot - Anti-Lck (phospho Y505) antibody [LckY505-A3] (ab278722)
    All lanes : Anti-Lck (phospho Y505) antibody [LckY505-A3] (ab278722) at 0.1 µg/ml

    All lanes : Jurkat cell extract treated with calyculin A

    Predicted band size: 58 kDa

  • Anti-Lck (phospho Y505) antibody [LckY505-A3] (ab278722)
    Anti-Lck (phospho Y505) antibody [LckY505-A3] (ab278722)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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