Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD83 antibody [3D11] - BSA and Azide free (ab255778)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [3D11] to CD83 - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD83 antibody [3D11] - BSA and Azide free
    See all CD83 primary antibodies

  • Description

    Rat monoclonal [3D11] to CD83 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    See all applications and species data

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Flow Cyt: Mouse splenocytes treated with LPS.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255778 is the carrier-free version of ab252822. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255778 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-CD83 antibody [3D11] - BSA and Azide free (ab255778)
    Flow Cytometry - Anti-CD83 antibody [3D11] - BSA and Azide free (ab255778)

    This data was developed using ab252822 the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of mouse splenocytes treated with 10µg/ml lipopolysaccharide (LPS) for 4h (Right) / Untreated control (Left) cells labelling CD83 with ab252822 at 1/1000 dilution (0.1µg). Goat F(ab)2 Anti-rat IgG Fc (Alexa Fluor® 488, ab150161 at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with ab252822. Then stained with anti-B220 conjugated to Alexa Fluor® 647. Gated on viable cells.

  • Anti-CD83 antibody [3D11] - BSA and Azide free (ab255778)
    Anti-CD83 antibody [3D11] - BSA and Azide free (ab255778)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.