Anti-CD300C antibody [EPR16470] - BSA and Azide free (ab251213)
![Anti-CD300C antibody [EPR16470] - BSA and Azide free (ab251213)](https://www.abcam.com/ps/products/251/ab251213/Images/ab251213-405741-anti-cd300c-antibody-epr16470-bsa-and-azide-free-western-blot.jpg "Anti-CD300C antibody [EPR16470] - BSA and Azide free (ab251213)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16470] to CD300C - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CD300C antibody [EPR16470] - BSA and Azide free
See all CD300C primary antibodies -
Description
Rabbit monoclonal [EPR16470] to CD300C - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251213 is the carrier-free version of ab195811. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251213 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16470 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD300C antibody [EPR16470] - BSA and Azide free (ab251213)All lanes : Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Rat heart tissue lysate
Lane 5 : Rat spleen tissue lysate
Lane 6 : RAW 264.7 cell lysate
Lane 7 : PC12 lysate
Lane 8 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 25 kDaThis data was developed using ab195811, the same antibody clone in a different buffer formulation.
The observed MW is consistent with what has been described in the literature (PMID:20656921).
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![Western blot - Anti-CD300C antibody [EPR16470] - BSA and Azide free (ab251213)](https://www.abcam.com/ps/products/251/ab251213/Images/ab251213-403459-anti-cd300c-antibody-epr16470-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CD300C antibody [EPR16470] - BSA and Azide free (ab251213)All lanes : Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 25 kDaThis data was developed using ab195811, the same antibody clone in a different buffer formulation.
The observed MW is consistent with what has been described in the literature (PMID:20656921).
-
![Western blot - Anti-CD300C antibody [EPR16470] - BSA and Azide free (ab251213)](https://www.abcam.com/ps/products/251/ab251213/Images/ab251213-399045-anti-cd300c-antibody-epr16470-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CD300C antibody [EPR16470] - BSA and Azide free (ab251213)Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution + THP1 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 25 kDaThis data was developed using ab195811, the same antibody clone in a different buffer formulation.
The observed MW is consistent with what has been described in the literature (PMID: 20656921).
-
![Anti-CD300C antibody [EPR16470] - BSA and Azide free (ab251213)](https://www.abcam.com/ps/products/251/ab251213/Images/ab251213-4-benefits-of-recombinant-antibodies.png)
Anti-CD300C antibody [EPR16470] - BSA and Azide free (ab251213)