Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD2 antibody [EPR21825] - BSA and Azide free (ab231059)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21825] to CD2 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD2 antibody [EPR21825] - BSA and Azide free
    See all CD2 primary antibodies

  • Description

    Rabbit monoclonal [EPR21825] to CD2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse thymus tissue.

  • General notes

    Ab231059 is the carrier-free version of ab219411. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab231059 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-CD2 antibody [EPR21825] - BSA and Azide free (ab231059)
    Flow Cytometry - Anti-CD2 antibody [EPR21825] - BSA and Azide free (ab231059)

    Flow cytometric analysis of mouse primary splenocytes labeling CD2 with ab219411 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were surface stained with anti-mouse CD19-Alexa Fluor®647 and rabbit IgG (left) / ab219411 (right). Gated on total viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219411).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] - BSA and Azide free (ab231059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] - BSA and Azide free (ab231059)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD2 with ab219411 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse spleen (PMID: 2901356) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219411).

  • Immunoprecipitation - Anti-CD2 antibody [EPR21825] - BSA and Azide free (ab231059)
    Immunoprecipitation - Anti-CD2 antibody [EPR21825] - BSA and Azide free (ab231059)

    CD2 was immunoprecipitated from 0.35 mg of mouse thymus lysate with ab219411 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219411 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: Mouse thymus lysate 10 µg (Input). 

    Lane 2: ab219411 IP in mouse thymus lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219411 in mouse thymus lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219411).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] - BSA and Azide free (ab231059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] - BSA and Azide free (ab231059)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD2 with ab219411 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse thymus (PMID: 2901356) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219411).

  • Anti-CD2 antibody [EPR21825] - BSA and Azide free (ab231059)
    Anti-CD2 antibody [EPR21825] - BSA and Azide free (ab231059)

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