Creatinine Assay Kit (Colorimetric) (ab204537)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Urine
Overview
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Product name
Creatinine Assay Kit (Colorimetric)
See all Creatinine kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 7.981 mg/dl 20 = 2.159% 7.452 mg/dl 20 = 3.203% 2.007 mg/dl 20 = 5.995% 7.326 mg/dl 20 = 3.729% Inter-assay Sample n Mean SD CV% 1.932 mg/dl 20 = 2.027% 7.085 mg/dl 20 = 1.433% 7.591 mg/dl 20 = 2.249% 7.232 mg/dl 20 = 2.052% -
Sample type
Urine -
Assay type
Quantitative -
Recovery
Sample specific recovery Sample type Average % Range Urine = 95 92% - 99% -
Assay time
0h 30m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's Creatinine Assay Kit (Colorimetric) (ab204537) is a complete kit for the quantitative determination of creatinine in urine, and is based upon the Jaffe reaction.
Sensitivity = 0.042 mg/dL.
General range = 0.31 - 20 mg/dL
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Notes
Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or is synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and p-creatine are converted nonenzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine, and under normal conditions, its formation occurs at a relatively constant rate. Intra-individual variation of creatinine levels is
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Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 2 x 96 tests Clear Microtiter Plates 2 units Creatinine Detection Reagent 1 x 20ml Creatinine Standard 1 vial Plate Sealer 2 units -
Research areas
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Relevance
Creatinine, or creatine anhydride, is a breakdown product of creatine phosphate in muscle. The loss of water molecule from creatine results in the formation of creatinine. Creatinine is transferred to the kidneys by blood plasma, whereupon it is eliminated from the body by glomerular filtration and partial tubular excretion. Creatinine is usually produced and excreted at a fairly constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function.
Images
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Typical standard curve obtained using Creatinine Assay Kit (Colorimetric) (ab204537).
Typical standard curve obtained using Creatinine Assay Kit (Colorimetric) (ab204537) which is a complete kit for the quantitative determination of creatinine in urine, and is based upon the Jaffe reaction.
