Creatinine Assay Kit (ab65340)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Urine
Overview
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Product name
Creatinine Assay Kit
See all Creatinine kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids -
Assay type
Quantitative -
Assay time
1h 00m -
Product overview
Creatinine Assay Kit ab65340 provides an accurate, convenient measure of creatinine concentration in biological fluids such as serum, urine or CSF.
In the creatinine assay protocol, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine, which is specifically oxidized to produce a product which reacts with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 538/587 nm).
Unlike picric acid assays, this kit is suitable for serum/plasma creatinine determinations, as well as for urine and other biological samples.
Creatinine assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 60 min at 37ºC
- analyze with microplate readerFor deproteinization of samples: Better results are typically seen with this assay when using a 10kda filter for sample deproteinization than when using the PCA method.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Creatinase (Lyophilized) Blue 1 vial Creatininase (Lyophilized) Violet 1 vial Creatinine Standard (10 µmol) (Lyophilized) Yellow 1 vial Creatinine Assay Buffer WM 1 x 25ml Creatinine Enzyme Mix (Lyophilized) Green 1 vial Creatinine Probe in DMSO (200ul) 1 x 200µl -
Research areas
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Relevance
Creatinine, or creatine anhydride, is a breakdown product of creatine phosphate in muscle. The loss of water molecule from creatine results in the formation of creatinine. Creatinine is transferred to the kidneys by blood plasma, whereupon it is eliminated from the body by glomerular filtration and partial tubular excretion. Creatinine is usually produced and excreted at a fairly constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function.
Images
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Functional Studies - Creatinine Assay Kit (ab65340) Gai, Zhibo et al., Scientific reports vol. 7,1 9815., Fig 2, doi:10.1038/s41598-017-10168-6Mice were injected i.p. with 6-ECDCA before I/R injury. At 24 h after I/R, kidney, serum and urine samples were collected for measurements of creatinine in serum with ab65340
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Functional Studies - Creatinine Assay Kit (ab65340)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - Creatinine Assay Kit (ab65340)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - Creatinine Assay Kit (ab65340)Creatinine measured in mouse and human serum plotted against RFU. Samples were diluted 5-10 fold.
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Functional Studies - Creatinine Assay Kit (ab65340)Creatinine measured in mouse and human urine plotted against RFU. Samples were diluted 400-800 fold.
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Immunodiffusion - Creatinine Assay Kit (ab65340)Creatinine levels in filtered human urine was measured in the presence or absence of creatininase (background signal subtracted.
