Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3148] to IKB alpha (phospho S32) - BSA and Azide free
  • Suitable for: IP, Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free
    See all IKB alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR3148] to IKB alpha (phospho S32) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: TNF-a treated HeLa and TNF-a treated MCF7 whole cell lysates; IP: TNF-a treated HeLa whole cell lysate.

  • General notes

    ab239920 is the carrier-free version of ab92700 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239920 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)
    Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)
    All lanes : Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700) at 1/1000 dilution (Purified)

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) treated with 20 ng/mL TNF-alpha for 8 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Blocking/Diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92700).

  • Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)
    Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)
    All lanes : Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20 ng/mL TNF-alpha for 5 minutes whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Blocking/Diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92700).

  • Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)
    Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)

    Purified ab92700 at 1:20 dilution (1μg) immunoprecipitating IKB alpha in HeLa treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate 10μg
    Lane 2 (+): ab92700 + HeLa treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92700 in HeLa treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 36 kDa

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92700).

  • Dot Blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)
    Dot Blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)

    Dot blot analysis of IKB alpha (phospho S32) phospho peptide (Lane 1) and IKB alpha non-phospho peptide (Lane 2) labeling IKB alpha (phospho S32) with unpurified ab92700 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/10000 was used as the secondary antibody.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92700).

  • Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)
    Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)

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