Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MYBBP1A antibody [EPR7205] (ab202896)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7205] to MYBBP1A
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-MYBBP1A antibody [EPR7205]
    See all MYBBP1A primary antibodies

  • Description

    Rabbit monoclonal [EPR7205] to MYBBP1A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, HeLa and Jurkat cell lysates. IHC-P: Human cerebral cortex and cervix carcinoma tissues. ICC/IF: SH-SY5Y and HeLa cells Flow Cyt: HeLa

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MYBBP1A antibody [EPR7205] (ab202896)
    Western blot - Anti-MYBBP1A antibody [EPR7205] (ab202896)
    Anti-MYBBP1A antibody [EPR7205] (ab202896) at 1/1000 dilution + HEK-293 (Human epithelial cells from embryonic kidney) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 149 kDa
    Observed band size: 149 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBBP1A antibody [EPR7205] (ab202896)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBBP1A antibody [EPR7205] (ab202896)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling MYBBP1A with ab202896 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MYBBP1A antibody [EPR7205] (ab202896)
    Immunocytochemistry/ Immunofluorescence - Anti-MYBBP1A antibody [EPR7205] (ab202896)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling MYBBP1A with ab202896 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and weakly cytoplasmic staining on SH-SY5Y cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202896 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Flow Cytometry - Anti-MYBBP1A antibody [EPR7205] (ab202896)
    Flow Cytometry - Anti-MYBBP1A antibody [EPR7205] (ab202896)

    Flow cytometry analysis of HeLa cells labelling MYBBP1A (red) with purified ab202896 at dilution of 1/70. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • Western blot - Anti-MYBBP1A antibody [EPR7205] (ab202896)
    Western blot - Anti-MYBBP1A antibody [EPR7205] (ab202896)
    Anti-MYBBP1A antibody [EPR7205] (ab202896) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 149 kDa
    Observed band size: 149 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBBP1A antibody [EPR7205] (ab202896)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBBP1A antibody [EPR7205] (ab202896)

    Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue labeling MYBBP1A with ab202896 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MYBBP1A antibody [EPR7205] (ab202896)
    Immunocytochemistry/ Immunofluorescence - Anti-MYBBP1A antibody [EPR7205] (ab202896)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling MYBBP1A with ab202896 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and weakly cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202896 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Western blot - Anti-MYBBP1A antibody [EPR7205] (ab202896)
    Western blot - Anti-MYBBP1A antibody [EPR7205] (ab202896)
    Anti-MYBBP1A antibody [EPR7205] (ab202896) at 1/1000 dilution + Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 149 kDa
    Observed band size: 149 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-MYBBP1A antibody [EPR7205] (ab202896)
    Anti-MYBBP1A antibody [EPR7205] (ab202896)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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