Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22641-16] to MAP2 - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free
    See all MAP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22641-16] to MAP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
    Unsuitable for: Flow Cyt,ICC/IF,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human cerebrum and colon tissue; mouse and rat cerebrum tissue. IHC-Fr: Mouse and rat cerebral cortex and cerebellum tissue.

  • General notes

    Ab256512 is the carrier-free version of ab254264. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256512 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)
    Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebral cortex tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on rat cerebral cortex (PMID: 22275884) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254264).

  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)
    Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebral cortex tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on mouse cerebral cortex (PMID: 22275884) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254264).

  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)
    Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on rat cerebellum (PMID: 22275884) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254264).

  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)
    Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on mouse cerebellum (PMID: 22275884) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254264).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in neuron of human colon (PMID: 28766965,15642108) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254264).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in rat cerebrum (PMID: 28766965,15642108) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254264).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in mouse cerebrum (PMID: 28766965,15642108) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254264).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human cerebrum (PMID: 28766965,15642108) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254264).

  • Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)
    Anti-MAP2 antibody [EPR22641-16] - BSA and Azide free (ab256512)

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