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Phosphotyrosine ErbB3 / HER3 ELISA Kit (ab279789)

Key features and details

  • Sample type: Cell Lysate
  • Detection method: Colorimetric
  • Assay type: Semi-quantitative
  • Reacts with: Human

Overview

  • Product name

    Phosphotyrosine ErbB3 / HER3 ELISA Kit
    See all ErbB3 / HER3 kits

  • Detection method

    Colorimetric

  • Sample type

    Cell Lysate

  • Assay type

    Semi-quantitative

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Phosphotyrosine ErbB3 / HER3 ELISA Kit (ab279789) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ErbB3 / HER3 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.


    This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-ErbB3 / HER3. An anti-pan ErbB3 / HER3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and ErbB3 / HER3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated ErbB3 / HER3. After washing away unbound antibody, HRP conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-ErbB3 / HER3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    2X Cell lysate buffer 1 x 10ml
    5X Assay Diluent 1 x 15ml
    600X HRP-Streptavidin Concentrate 1 vial
    Biotinylated anti-phosphotyrosine antibody 2 vials
    Pan-ErbB3 / HER3 Coated Microplate 1 unit
    Positive Control - A431 cell lysate 1 vial
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • Research areas

  • Function

    Binds and is activated by neuregulins and NTAK.

  • Tissue specificity

    Epithelial tissues and brain.

  • Involvement in disease

    Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.

  • Developmental stage

    Overexpressed in a subset of human mammary tumors.

  • Domain

    The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.

  • Post-translational
    modifications

    Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Alternative names

    • c erbB 3
    • c erbB3
    • Erb b2 receptor tyrosine kinase 3
    • ErbB 3
    • ERBB3
    • ERBB3 protein
    • erbB3 S
    • ERBB3_HUMAN
    • Glial growth factor receptor
    • HER 3
    • HER3
    • Human epidermal growth factor receptor 3
    • LCCS2
    • MDA BF 1
    • MGC88033
    • p180 ErbB3
    • p45 sErbB3
    • p85 sErbB3
    • proto-oncogene-like protein c ErbB 3
    • proto-oncogene-like protein c ErbB3
    • Proto-oncogene-like protein c-ErbB-3
    • Receptor tyrosine protein kinase erbB 3
    • Receptor tyrosine protein kinase erbB3
    • Receptor tyrosine-protein kinase erbB-3
    • Tyrosine kinase type cell surface receptor HER3
    • Tyrosine kinase-type cell surface receptor HER3
    • v erb b2 avian erythroblastic leukemia viral oncogene homolog 3
    • v erb b2 erythroblastic leukemia viral oncogene homolog 3
    • v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
    see all

  • Database links

    Images

    • Positive Control
      Positive Control

      A431 cells were treated with recombinant human EGF at 37°C for 10 min.

      Solubilize cells at 4 x 107 cells/ml in lysis buffer.

      Serial dilutions of lysates were analyzed in this ELISA.

    • A431 cells treated with/without EGF.
      A431 cells treated with/without EGF.
      A431 cells were untreated or treated with 100 ng/ml recombinant human EGF for 10 mins.

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