Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)
![Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)](https://www.abcam.com/ps/products/255/ab255607/Images/ab255607-5-anti-erbb-3-antibody-epr22669-25-western-blot-human.jpg "Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22669-25] to ErbB3 / HER3
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
Overview
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Product name
Anti-ErbB3 / HER3 antibody [EPR22669-25]
See all ErbB3 / HER3 primary antibodies -
Description
Rabbit monoclonal [EPR22669-25] to ErbB3 / HER3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P MouseHumanWB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7, 4T1, SK-BR-3 and T-47D whole cell lysate. Mouse stomach and skin tissue lysate. IHC-P: Human bladder cancer and breast cancer tissue. Mouse skin tissue. Flow Cyt: MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22669-25 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)All lanes : Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 148 kDa
Exposure time: 26 secondsFresh lysates are needed for western blot.
ErbB 3 low expression cell lines: HeLa, MDA-MB-231 (PMID: 22436610, 22301547).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)](https://www.abcam.com/ps/products/255/ab255607/Images/ab255607-2-anti-erbb-3-antibody-epr22669-25-immunocytochemistry-bladder-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling ErbB3 / HER3 with ab255607 at 1/250 dilution (2.1 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining in human urothelial carcinoma (PMID: 23333248) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
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![Flow Cytometry - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)](https://www.abcam.com/ps/products/255/ab255607/Images/ab255607-1-anti-erbb-3-antibody-epr22669-25-flowcytometry-mcf7-mdamb231-human.jpg)
Flow Cytometry - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MDA-MB-231 (Human breast adenocarcinoma epithelial cell, Left) / MCF7 (Human breast adenocarcinoma epithelial cell, Right) cells labeling ErbB3 / HER3 with ab255607 at 1/50 dilution (1μg)/ Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Weak expression control: MDA-MB-231 (PMID: 25018110, 16519802).
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![Western blot - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)](https://www.abcam.com/ps/products/255/ab255607/Images/ab255607-7-anti-erbb-3-antibody-epr22669-25-western-blot.jpg)
Western blot - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)All lanes : Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607) at 1/1000 dilution
Lane 1 : 4T1 (Mouse mammary gland carcinoma epithelial cell) whole cell lysate
Lane 2 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 148 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)](https://www.abcam.com/ps/products/255/ab255607/Images/ab255607-4-anti-erbb-3-antibody-epr22669-25-immunocytochemistry-skin-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling ErbB3 / HER3 with ab255607 at 1/250 dilution (2.1 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining in mouse skin (PMID:26194695) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
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![Western blot - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)](https://www.abcam.com/ps/products/255/ab255607/Images/ab255607-6-anti-erbb-3-antibody-epr22669-25-western-blot-mouse.jpg)
Western blot - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)All lanes : Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607) at 1/1000 dilution
Lane 1 : Mouse stomach lysate
Lane 2 : Mouse skin lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 148 kDa
Exposure time: 70 secondsThe expression profile/molecular weight observed is consistent with what has been described in the literature. PMID: 26194695 and 19690388.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)](https://www.abcam.com/ps/products/255/ab255607/Images/ab255607-3-anti-erbb-3-antibody-epr22669-25-immunocytochemistry-breast-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling ErbB3 / HER3 with ab255607 at 1/250 dilution (2.1 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining in human breast carcinoma (PMID: 24346286) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
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![Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)](https://www.abcam.com/ps/products/255/ab255607/Images/ab255607-8-benefits-of-recombinant-antibodies.png)
Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)
