Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22669-25] to ErbB3 / HER3
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-ErbB3 / HER3 antibody [EPR22669-25]
See all ErbB3 / HER3 primary antibodies
Description

Rabbit monoclonal [EPR22669-25] to ErbB3 / HER3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF7, 4T1, SK-BR-3 and T-47D whole cell lysate. Mouse stomach and skin tissue lysate. IHC-P: Human bladder cancer and breast cancer tissue. Mouse skin tissue. Flow Cyt: MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22669-25
Isotype

IgG
Research areas

Western blot - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)

All lanes : Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607) at 1/1000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 148 kDa

Exposure time: 26 seconds

Fresh lysates are needed for western blot.

ErbB 3 low expression cell lines: HeLa, MDA-MB-231 (PMID: 22436610, 22301547).

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling ErbB3 / HER3 with ab255607 at 1/250 dilution (2.1 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining in human urothelial carcinoma (PMID: 23333248) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Flow Cytometry - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MDA-MB-231 (Human breast adenocarcinoma epithelial cell, Left) / MCF7 (Human breast adenocarcinoma epithelial cell, Right) cells labeling ErbB3 / HER3 with ab255607 at 1/50 dilution (1μg)/ Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Weak expression control: MDA-MB-231 (PMID: 25018110, 16519802).
Western blot - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)

All lanes : Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607) at 1/1000 dilution

Lane 1 : 4T1 (Mouse mammary gland carcinoma epithelial cell) whole cell lysate
Lane 2 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 148 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling ErbB3 / HER3 with ab255607 at 1/250 dilution (2.1 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining in mouse skin (PMID:26194695) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Western blot - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)

All lanes : Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607) at 1/1000 dilution

Lane 1 : Mouse stomach lysate
Lane 2 : Mouse skin lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 148 kDa

Exposure time: 70 seconds

The expression profile/molecular weight observed is consistent with what has been described in the literature. PMID: 26194695 and 19690388.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling ErbB3 / HER3 with ab255607 at 1/250 dilution (2.1 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining in human breast carcinoma (PMID: 24346286) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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