Anti-ErbB3 / HER3 antibody (ab226260)
Key features and details
- Rabbit polyclonal to ErbB3 / HER3
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ErbB3 / HER3 antibody
See all ErbB3 / HER3 primary antibodies -
Description
Rabbit polyclonal to ErbB3 / HER3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human ErbB3/ HER3 aa 1275-1325. The exact sequence is proprietary. (NP_001973.2).
Database link: P21860 -
Positive control
- IP: MCF7 whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226260 was affinity purified using an epitope specific to ErbB 3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-ErbB3 / HER3 antibody (ab226260)
ErbB 3 was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell lysate (prepared using RIPA buffer; 1 mg for IP, 20% of IP loaded) with ab226260 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different rabbit anti-ErbB 3 antibody at 1 µg/ml.
Lane 1: ab226260 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
