Anti-Histone H3 (mono methyl K37) antibody (ab272160)
Key features and details
- Rabbit polyclonal to Histone H3 (mono methyl K37)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (mono methyl K37) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (mono methyl K37) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IF HumanWB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (mono methyl K37).
Database link: Q71DI3 -
Positive control
- WB: HeLa, NIH/3T3, Elegans embryo lysate. ICC/IF: HeLa DotBlot: K37, K37A, K37Me1, K37Me2, K37Me3, K37me1, K36me1, K36me2, K36me3, K36ac
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.004% Potassium phosphate, 0.0008% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 antibody (ab272160)All lanes : Anti-Histone H3 (mono methyl K37) antibody (ab272160) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : NIH/3T3 cell lysate
Lane 3 : Elegans embryo lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : RDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT at 1/10000 dilution
Predicted band size: 15 kDaBlock: 5% BLOTTO overnight at 4°C.
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Dot Blot - Anti-Histone H3 antibody (ab272160)Dot Blot analysis of ab272160.
Lane 1: K37me1.
Lane 2: K36me1.
Lane 3: K36me2.
Lane 4: K36me3.
Lane 5: K36ac.
Load: 1, 10, and 100 picomoles of peptide. Primary antibody: ab272160 at 1:1000 for 45 minutes at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10,000 for 45 minutes at RT. Block: 5% BLOTTO overnight at 4°C.
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Dot Blot - Anti-Histone H3 antibody (ab272160)Dot Blot analysis of ab272160.
Lane 1: K37.
Lane 2: K37Ac.
Lane 3: K37Me1.
Lane 4: K37Me2.
Lane 5: K37Me3.
Load: 1, 10, and 100 picomoles of peptide. Primary antibody: ab272160 at 1:1000 for 45 minutes at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10,000 for 45 minutes at room temperature. Block: 5% BLOTTO overnight at 4°C.
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Immunofluorescence - Anti-Histone H3 (mono methyl K37) antibody (ab272160)Immunofluorescence analysis of Anti-Histone H3K37me. ab272160 was tested at 1:200 in HeLa cells with FITC (green). Cells and nuclei were counterstained with DAPI (blue) and DyLight 550 (red). (40X)