Anti-Histone H3 (acetyl K23) antibody (ab61234)
Key features and details
- Rabbit polyclonal to Histone H3 (acetyl K23)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (acetyl K23) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (acetyl K23) -
Host species
Rabbit -
Specificity
ab61234 detects endogenous levels of Histone H3 only when acetylated at lysine 23 (Human: Lys23; Mouse: Lys23; Rat: Lys23). -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (acetyl K23).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab61234 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Histone H3 (acetyl K23) antibody (ab61234) at 1/500 dilution
Lane 1 : Extracts from RAW264.7 cells
treated with TSA (400nM, 24hours)
Lane 2 : Extracts from RAW264.7 cells
treated with TSA (400nM, 24hours) with the immunizing acetylated peptide
Predicted band size: 15 kDa
Observed band size: 15 kDa
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Immunofluorescence analysis of HeLa cells, using Histone H3 (acetyl K23) antibody (ab61234) at 1/100 - 1/500 dilution, in the presence (right panel) or absence (left panel) of immunizing acetylated peptide.