Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD161 antibody [EPR23831-120] (ab259916)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23831-120] to CD161
  • Suitable for: ICC, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD161 antibody [EPR23831-120]
    See all CD161 primary antibodies

  • Description

    Rabbit monoclonal [EPR23831-120] to CD161

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: Human PBMC cells. Flow Cyt: Human PBMC cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23831-120

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry - Anti-CD161 antibody [EPR23831-120] (ab259916)
    Immunocytochemistry - Anti-CD161 antibody [EPR23831-120] (ab259916)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC cells labelling CD161 with ab259916 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in subsets of human PBMC cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-CD161 antibody [EPR23831-120] (ab259916)
    Flow Cytometry - Anti-CD161 antibody [EPR23831-120] (ab259916)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD161 with ab259916 at 1/500 dilution/ Right compared with a Rabbit monoclonal IgG isotype control (ab172730) / Left. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG or ab259916. Then stained with anti-CD4 conjugated to Alexa Fluor® 647.

    Gated on viable cells.

  • Flow Cytometry - Anti-CD161 antibody [EPR23831-120] (ab259916)
    Flow Cytometry - Anti-CD161 antibody [EPR23831-120] (ab259916)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD161 with ab259916 at 1/500 dilution/ Right compared with a Rabbit monoclonal IgG isotype control (ab172730) / Left. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG or ab259916. Then stained with anti-CD4 conjugated to Alexa Fluor® 647 and anti-CD56 conjugated to BV421.

    Gated on viable CD4(-) population.

  • Flow Cytometry - Anti-CD161 antibody [EPR23831-120] (ab259916)
    Flow Cytometry - Anti-CD161 antibody [EPR23831-120] (ab259916)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD161 with ab259916 at 1/500 dilution/ Right compared with a Rabbit monoclonal IgG isotype control (ab172730) / Left. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG or ab259916. Then stained with anti-CD4 conjugated to Alexa Fluor® 647 and anti-CD56 conjugated to BV421.

    Gated on viable CD56(-) population.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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