Recombinant Human HCST protein (Fc Chimera His Tag) (ab229357)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant Human HCST protein (Fc Chimera His Tag) -
Purity
> 90 % SDS-PAGE.
ab229357 was purified by using conventional chromatography techniques. -
Endotoxin level
Expression system
Baculovirus infected insect cellsAccession
Protein length
Protein fragmentAnimal free
NoNature
Recombinant-
Species
Human -
Sequence
ADPTTPGERSSLPAFYPGTSGSCSGCGSLSLPLEPKSCDKTHTCPPCPAP ELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGV EVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWE SNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEAL HNHYTQKSLSLSPGKHHHHHH -
Predicted molecular weight
30 kDa including tags -
Amino acids
20 to 48 -
Tags
His tag C-Terminus -
Additional sequence information
Fused to hIgG-His tag at the C-terminus (NP_055081).
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab229357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- DAP10
- DNAX activation protein 10
- Hematopoietic cell signal transducer
see all -
Relevance
HCST is a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. It may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D and may activate phosphatidylinositol 3-kinase dependent signaling pathways. The receptor complex containing HCST may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms. -
Cellular localization
Cell Membrane
Images
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SDS-PAGE - Recombinant Human HCST protein (Fc Chimera His Tag) (ab229357)
15% SDS-PAGE analysis of 3 µg ab229357.
MW: 28-40 kDa (SDS-PAGE under reducing conditions).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab229357 has not yet been referenced specifically in any publications.
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Images
-
SDS-PAGE - Recombinant Human HCST protein (Fc Chimera His Tag) (ab229357)
15% SDS-PAGE analysis of 3 µg ab229357.
MW: 28-40 kDa (SDS-PAGE under reducing conditions).