Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 117 264 ₸

Order now and get it on Wednesday March 03, 2021

Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2541Y] to WASP/Wiskott-Aldrich syndrome protein
Suitable for: WB, IHC-P, Flow Cyt, ICC
Reacts with: Human

Product name

Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y]
See all WASP/Wiskott-Aldrich syndrome protein primary antibodies
Description

Rabbit monoclonal [EP2541Y] to WASP/Wiskott-Aldrich syndrome protein
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: TF-1 and U-937 whole cell lysates; ICC: TF-1 cells; Flow Cyt: U-937 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as WASP

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP2541Y
Isotype

IgG
Research areas

Immunocytochemistry - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)

Immunocytochemistry analysis of TF-1 (Human bone marrow erythroblast) cells labeling WASP/Wiskott-Aldrich syndrome protein with Purified ab75830 at 1:50 dilution (5.88 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)

Flow Cytometry analysis of U-937 (Human histiocytic lymphoma monocyte) cells labeling WASP/Wiskott-Aldrich syndrome protein with Purified ab75830 at 1:30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)

All lanes : Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830) at 1/10000 dilution (Purified)

Lane 1 : TF-1 (Human Erythroleukemia erythroblast) whole cell lysate
Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 53 kDa
Observed band size: 60 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com