Antibodies, Proteins, Kits and Reagents for Life Science

Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (ab202025)

Key features and details

Mouse monoclonal [7B10E4] to WASP/Wiskott-Aldrich syndrome protein
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human
Isotype: IgG2a

Product name

Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4]
See all WASP/Wiskott-Aldrich syndrome protein primary antibodies
Description

Mouse monoclonal [7B10E4] to WASP/Wiskott-Aldrich syndrome protein
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human WASP/Wiskott-Aldrich syndrome protein aa 50-200. Expressed in E. Coli.
Database link: P42768

Run BLAST with Run BLAST with
Positive control
- Human WASP/Wiskott-Aldrich syndrome protein (aa: 57-170) recombinant protein; Human WASP/Wiskott-Aldrich syndrome protein (aa: 57-170)-hIgGFc transfected HEK293 cell lysate; HeLa cells; Human ovarian cancer and colon cancer tissues
General notes

This product was previously labelled as WASP

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

7B10E4
Isotype

IgG2a
Research areas

Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (ab202025)

Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (ab202025) at 1/500 dilution + Human WASP/Wiskott-Aldrich syndrome protein (aa: 57-170) recombinant protein at 1 µg

Predicted band size: 53 kDa

Expected MW is 39 kDa
Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (ab202025)

All lanes : Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (ab202025) at 1/500 dilution

Lane 1 : Non transfected HEK293 cell lysate
Lane 2 : Human WASP/Wiskott-Aldrich syndrome protein (aa: 57-170)-hIgGFc transfected HEK293 cell lysate

Lysates/proteins at 1 µg per lane.

Predicted band size: 53 kDa
Flow Cytometry - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (ab202025)

Flow cytometric analysis of HeLa cells labeling WASP/Wiskott-Aldrich syndrome protein using ab202025 at 1/200 dilution (green) and negative control (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (ab202025)

Immunohistochemical analysis of paraffin embedded Human ovarian cancer tissues labeling WASP/Wiskott-Aldrich syndrome protein using ab202025 at 1/200 dilution, followed by DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (ab202025)

Immunohistochemical analysis of paraffin embedded Human colon cancer tissues labeling WASP/Wiskott-Aldrich syndrome protein using ab202025 at 1/200 dilution, followed by DAB staining.

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