Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (ab202025)
Key features and details
- Mouse monoclonal [7B10E4] to WASP/Wiskott-Aldrich syndrome protein
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4]
See all WASP/Wiskott-Aldrich syndrome protein primary antibodies -
Description
Mouse monoclonal [7B10E4] to WASP/Wiskott-Aldrich syndrome protein -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human WASP/Wiskott-Aldrich syndrome protein aa 50-200. Expressed in E. Coli.
Database link: P42768 -
Positive control
- Human WASP/Wiskott-Aldrich syndrome protein (aa: 57-170) recombinant protein; Human WASP/Wiskott-Aldrich syndrome protein (aa: 57-170)-hIgGFc transfected HEK293 cell lysate; HeLa cells; Human ovarian cancer and colon cancer tissues
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General notes
This product was previously labelled as WASP
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One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
7B10E4 -
Isotype
IgG2a -
Research areas
Images
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Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (ab202025) at 1/500 dilution + Human WASP/Wiskott-Aldrich syndrome protein (aa: 57-170) recombinant protein at 1 µg
Predicted band size: 53 kDaExpected MW is 39 kDa
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All lanes : Anti-WASP/Wiskott-Aldrich syndrome protein antibody [7B10E4] (ab202025) at 1/500 dilution
Lane 1 : Non transfected HEK293 cell lysate
Lane 2 : Human WASP/Wiskott-Aldrich syndrome protein (aa: 57-170)-hIgGFc transfected HEK293 cell lysate
Lysates/proteins at 1 µg per lane.
Predicted band size: 53 kDa
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Flow cytometric analysis of HeLa cells labeling WASP/Wiskott-Aldrich syndrome protein using ab202025 at 1/200 dilution (green) and negative control (red).
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Immunohistochemical analysis of paraffin embedded Human ovarian cancer tissues labeling WASP/Wiskott-Aldrich syndrome protein using ab202025 at 1/200 dilution, followed by DAB staining.
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Immunohistochemical analysis of paraffin embedded Human colon cancer tissues labeling WASP/Wiskott-Aldrich syndrome protein using ab202025 at 1/200 dilution, followed by DAB staining.