Anti-DDT antibody (ab191050)
Key features and details
- Rabbit polyclonal to DDT
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDT antibody
See all DDT primary antibodies -
Description
Rabbit polyclonal to DDT -
Host species
Rabbit -
Specificity
No cross reactivity with other proteins. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human DDT aa 1 to the C-terminus. E. coli derived.
Database link: P30046
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDT antibody (ab191050)
DDT was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab191050 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Western blot - Anti-DDT antibody (ab191050)Anti-DDT antibody (ab191050) at 0.5 µg/ml + Recombinant Human DDT Protein at 0.5 µg
Secondary
goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 13 kDa
