Anti-DDT antibody (ab115785)
Key features and details
- Rabbit polyclonal to DDT
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DDT antibody -
Description
Rabbit polyclonal to DDT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to a sequence at the C-terminal of Human DDT.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDT antibody (ab115785)
DDT was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab115785 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex DAB as the chromogen.
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Western blot - Anti-DDT antibody (ab115785)All lanes : Anti-DDT antibody (ab115785) at 0.5 µg/ml
Lane 1 : rat liver tissues lysate
Lane 2 : rat testis tissues lysate
Lanes 3-4 : mouse liver tissues lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 13 kDa
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Western blot - Anti-DDT antibody (ab115785)All lanes : Anti-DDT antibody (ab115785) at 1 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat pancreas tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : Rat lung tissue lysate
Predicted band size: 13 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDT antibody (ab115785)ab115785 at 1µg/ml staining DTT in Paraffin-embedded Human placenta tissue by Immunohistochemistry.
