Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-htrA1 antibody [EPR23240-64] (ab274322)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23240-64] to htrA1
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-htrA1 antibody [EPR23240-64]
    See all htrA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23240-64] to htrA1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SK-OV-3 whole cell lysate; Human brain, placenta, ovary and ovary cancer tissue lysates. IP: Human placenta tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23240-64

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322)
    Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322)
    All lanes : Anti-htrA1 antibody [EPR23240-64] (ab274322) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human placenta tissue lysate
    Lane 3 : Human ovary tissue lysate
    Lane 4 : Human ovary cancer tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 16767218, 19103920).

    Exposure time: 48 seconds.

  • Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] (ab274322)
    Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] (ab274322)

    htrA1 was immunoprecipitated from 0.35 mg Human placenta lysate with ab274322 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab274322 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human placenta lysate 10 ug

    Lane 2: ab274322 IP in Human placenta lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab274322 in Human placenta lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 7.75 seconds.

     

  • Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322)
    Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322)
    All lanes : Anti-htrA1 antibody [EPR23240-64] (ab274322) at 1/1000 dilution

    Lane 1 : SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: HepG2, MCF7 (PMID: 14716297, 22761798).

    Exposure time: 3 minutes. 

  • Anti-htrA1 antibody [EPR23240-64] (ab274322)
    Anti-htrA1 antibody [EPR23240-64] (ab274322)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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