Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZNF365 antibody [EPR10216] - BSA and Azide free (ab249328)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10216] to ZNF365 - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ZNF365 antibody [EPR10216] - BSA and Azide free
    See all ZNF365 primary antibodies

  • Description

    Rabbit monoclonal [EPR10216] to ZNF365 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, Flow Cyt, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249328 is the carrier-free version of ab157459. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249328 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR10216

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ZNF365 antibody [EPR10216] - BSA and Azide free (ab249328)
    Western blot - Anti-ZNF365 antibody [EPR10216] - BSA and Azide free (ab249328)
    All lanes : Anti-ZNF365 antibody [EPR10216] (ab157459) at 1/1000 dilution

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 24 kDa



    This data was developed using ab157459, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-ZNF365 antibody [EPR10216] - BSA and Azide free (ab249328)
    Immunocytochemistry - Anti-ZNF365 antibody [EPR10216] - BSA and Azide free (ab249328)
    This data was developed using ab157459, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of Jurkat cells labeling ZNF365 with ab157459 at 1/50 dilution.
  • Flow Cytometry - Anti-ZNF365 antibody [EPR10216] - BSA and Azide free (ab249328)
    Flow Cytometry - Anti-ZNF365 antibody [EPR10216] - BSA and Azide free (ab249328)
    This data was developed using ab157459, the same antibody clone in a different buffer formulation.Flow cytometic analysis of permeabilized Jurkat cells labeling ZNF365 with ab157459 at 1/10 dilution (red) or a rabbit IgG negative (green).
  • Immunoprecipitation - Anti-ZNF365 antibody [EPR10216] - BSA and Azide free (ab249328)
    Immunoprecipitation - Anti-ZNF365 antibody [EPR10216] - BSA and Azide free (ab249328)

    This data was developed using ab157459, the same antibody clone in a different buffer formulation.

    Detection of ZNF365 by Western Blot of Immunprecipitate. Jurkat cell lysate immunoprecipitated using ab157459 at 1/10 dilution.



    Anti-ZNF365 antibody [EPR10216] (ab157459) at 1/1000 dilution + Immunoprecipitation pellet from Jurkat cell lysate at 10 µg
  • Anti-ZNF365 antibody [EPR10216] - BSA and Azide free (ab249328)
    Anti-ZNF365 antibody [EPR10216] - BSA and Azide free (ab249328)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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