Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CPE antibody [EPR23306-21] - BSA and Azide free (ab275891)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23306-21] to CPE - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CPE antibody [EPR23306-21] - BSA and Azide free
    See all CPE primary antibodies

  • Description

    Rabbit monoclonal [EPR23306-21] to CPE - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
    Unsuitable for: Flow Cyt,ICC,IP or WB

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human and mouse pancreas tissue. IHC-Fr: Mouse pancreas tissue.

  • General notes

    ab275891 is the carrier-free version of ab275882. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275891 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPE antibody [EPR23306-21] - BSA and Azide free (ab275891)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPE antibody [EPR23306-21] - BSA and Azide free (ab275891)

    This data was developed using ab275882, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CPE with ab275882 at 1/2000 (0.266 µg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human pancreas islets (PMID:21152387). The section was incubated with ab275882 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-CPE antibody [EPR23306-21] - BSA and Azide free (ab275891)
    Immunohistochemistry (Frozen sections) - Anti-CPE antibody [EPR23306-21] - BSA and Azide free (ab275891)

    This data was developed using ab275882, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling CPE with ab275882 at 1/100 (5.31 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Green). Positive staining on mouse pancreatic islet is observed.  The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPE antibody [EPR23306-21] - BSA and Azide free (ab275891)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPE antibody [EPR23306-21] - BSA and Azide free (ab275891)

    This data was developed using ab275882, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling CPE with ab275882 at 1/2000 (0.266 µg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse islets is observed (PMID:21152387). The section was incubated with ab275882 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-CPE antibody [EPR23306-21] - BSA and Azide free (ab275891)
    Anti-CPE antibody [EPR23306-21] - BSA and Azide free (ab275891)

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