Anti-c-Myb antibody (ab177510)
Key features and details
- Rabbit polyclonal to c-Myb
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-c-Myb antibody
See all c-Myb primary antibodies -
Description
Rabbit polyclonal to c-Myb -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP Human
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Immunogen
Synthetic peptide within Human c-Myb aa 175-225. The exact sequence is proprietary. (NP_005366.2)
Sequence:GRTDNA IKNHWNSTMR RKVEQEGYLQ ESSKASQPAV ATSFQKNSHL MGFAQ
Database link: P10242 -
Positive control
- Human breast carcinoma tissue; MOLT4 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab177510 was affinity purified using an epitope specific to c-Myb immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-c-Myb antibody (ab177510)
Detection of Human c-Myb by Western Blot of Immunoprecipitate.
Lane 1: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MOLT4 cells.
Lane 2: Control IgG.
ab177510 used for IP at 6 μg/mg lysate.
ab177510 was used at 1 μg/ml for WB.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab177510)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling c-Myb with ab177510 at 1µg/ml. Detection:DAB.
